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120 samples of cord blood for F2-IsoPs, AOPP, and NPBI were examined.
 for NPBI with HPLC-DAD system (Agilent 1100 series), and the spectrophotometric method of Witko-Sarsat et al.
The indirect method of sampling suggested by Horn and Pesce  was used to estimate IRs for F2-IsoP, NPBI, and AOPP in cord blood sample of newborn.
In the multivariable analysis, using the cumulative concentration starting directly after surgery to 60 hours after surgery for each biomarker, correcting for GA, none of the potentially influencing factors showed a significant linear relation with plasma and urinary F2-isoprostane or plasma NPBI. Parameters investigated consisted of gender, type of congenital anomaly, endoscopic procedure, and the occurrence of parenchymal injury (Figure 4).
A significant difference in plasma NPBI was found between patients with nonparenchymal injury (78.5%) and patients with no brain injury after anaesthesia.
In the total patient cohort, after correcting for gestational age, perioperative biomarker concentrations of [F.sub.2]-isoprostane and NPBI were not correlated to perioperative brain injury.
Lipid peroxidation events in RTT skin fibroblasts were found to be related to the levels of NPBI, a prooxidant agent.
NPBI is a prooxidant factor, associated with hypoxia, hemoglobin oxidation, and subsequent heme iron release .
Generally, NPBI is considered not only an OS marker but a prooxidant factor.
The results of the redox and antioxidant markers in RTT patients showed significantly increased plasma levels of non-protein-bound iron (NPBI) (~2-fold), [F.sub.2]-isoprostanes ([F.sub.2]-IsoPs) (~2.9-fold), reduced glutathione (GSH) (~1.4-fold), oxidized glutathione (GSSG) (~50-fold), and intraerythrocyte NPBI (IE-NPBI) (~1.5-fold) as compared to healthy control subjects (Table 3).
Plasma was used for free isoprostanes ([F.sub.2]-isoprostanes, [F.sub.2]-IsoPs, and [F.sub.4]-neuroprostanes, [F.sub.4]-NeuroPs), 4-hydroxnonenal protein adducts (4-HNE PAs), and NPBI determinations.
Intraerythrocyte and Plasma NPBI. NPBI is a pro-oxidant factor, associated with hypoxia, hemoglobin oxidation, and subsequent heme iron release .
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