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Frequency of samples, AFP cases, contacts, cVDPVs isolated, NPEV cases, and Sabin-like poliovirus cases in Guinea, 2012-2016 * Year No.
Several years ago, my colleagues and I prospectively studied 380 children aged 4-18 years with systemic NPEV syndromes who presented to private suburban pediatric practices.
These results confirm those of a previous Tunisian epidemiological study of NPEV done with clinical samples (Bahri et al., 2005).
In a study my colleagues and I conducted a few years ago, only 8% of 372 children with a clinical diagnosis of systemic NPEV syndrome presented with HFM blisters.
"Now we're discovering that NPEV infections last longer, cost more, are more contagious, and present with a wider range of clinical syndromes than previously thought," said Dr.
Partial sequencing viral protein (VP1) was used to identify the NPEV (sero) types as described previously (14).
Of the 780 fecal specimens from apparently healthy children, [approximately not equal to] 20 (2.6%) fecal samples were positive for NPEV in cell culture, and [approximately not equal to] 23 (3.0%) were RT-PCR-positive with the same primers used for AFP samples (Table).
Number of specimens and poliovirus (PV) isolates, percentage of specimens with nonpolio enterovirus (NPEV) isolates, and timing of results, by World Health Organization (WHO) region and year, January 2003-June 2004 January-December 2003 % specimens No.
However, because the isolation rates of nonpolio enterovirus (NPEV) and Sabin virus from environmental samples demonstrated unexplained gradual decreases since late 2003, recent environmental surveillance data should be considered with caution.
The proportion of specimens with nonpolio enterovirus (NPEV) isolated, an indicator of the quality of stool-specimen transport and sensitivity of laboratory testing, was 22% and 25% in 2003 for Afghanistan and Pakistan, respectively; NPEV isolation rates during January-May 2004 were 19% for each country (Table).
The proportion of specimens with nonpolio enterovirus (NPEV) isolated is used as a combined indicator of quality of specimen transport and sensitivity of laboratory processing; a rate of >10% is considered acceptable.
The laboratory network's high performance level and virus detection sensitivity as measured through NPEV isolation rates ensure that poliovirus probably would be detected if present in specimens collected through the AFP surveillance system.
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