9] The expression levels of Type I and III procollagen proteins were significantly lower in the POP group compared with the NPOP group, whereas the opposite was noted for the expression of Mfn2 , and the previous results were published in European Journal of Obstetrics, Gynecology, and Reproductive Biology in 2014.
NPOP patients who accepted hysterectomy due to a benign gynecological disease ( n = 10) were enrolled as the control group [Table 1].
The data obtained by microscopical evaluation indicated that fibroblasts in the POP group exhibited lower adhesion numbers, survival rate, and smaller cell size, whereas those in the NPOP group indicated higher cell numbers and faster proliferation rates.
During the period of 5-11 days following seeding, the cells were in the logarithmic phase of proliferation, whereas the viability of the fibroblasts from the POP group was significantly lower compared with that of the NPOP group ( t [sub]d5, 7, 9, 11= −5.
The mRNA and protein expression levels of Mfn2 in the cultured POP fibroblasts were significantly higher compared with those of the NPOP group (mRNA: t = 2.
A previous study conducted by our group demonstrated the successful isolation of fibroblasts from the uterosacral ligament tissues of POP and NPOP patients using laser capture microdissection.
In addition, in terms of the enrollment criteria for the control group, only NPOP patients with a matched age, BMI, age of first delivery, number of deliveries, and postmenopausal duration who accepted hysterectomy due to a benign gynecologic disease were enrolled.
In contrast to these observations, the protein expression levels of both Type I and Type III procollagens in the in vitro cultured POP fibroblasts were lower compared with those of the NPOP fibroblasts, which was consistent with the results observed in the tissue study.