Western blot analysis of antioxidant proteins (Nrf-2/Keap-1, HO-1, and NQO1
We selected the NAD(P) H gene of quinone oxidoreductase 1 (NQO1
), as the induction of NQO1
gene expression occurs through the Nrf2-Keap1/ARE pathway.
High through put library screening identifies two novel NQO1
inducers in human lung cells.
Dash et al., "Genetic polymorphisms in NQO1
and SOD2: interactions with smoking, schistosoma infection, and bladder cancer risk in Egypt," Urologic Oncology: Seminars and Original Investigations, vol.
Triptolide (50-100 nM) is able to decrease mitochondrial respiration and increase ROS and apoptosis in p53-deficient non-small-cell lung cancer and consequently to upregulate Nrf2 and its target gene HO-1 and NQO1
 Nonstandard abbreviations: OMIM, Online Mendelian Inheritance in Man; TCA, tricarboxylic acid; IDH, isocitrate dehydrogenase; SDH, succinate dehydrogenase; FH, fumarate hydratase; PHD, prolyl hydroxylase domain protein; HIF, hypoxia-inducible factor; NRF2, nuclear factor (erythroid-derived 2)-like 2; NQO1
, NAD(P)H:quinone oxidoreductase 1.
In the MA sensitizer chemical group, KEAP1 and NRF2 were found as key hubs as well as their target genes NQO1
and HMOX1 (Ade et al., 2009; Natsch, 2010) and AHR (Schulz et al., 2013; Kohle and Bock, 2007).
As shown in Figure 3A, 1 [micro]M DL-Sulforaphane significantly induced the expression of NQO1
and SPP1 expression.
Garg and Maru  indicated that curcumin increased ARE-binding of Nrf2 and induced the activity as well as expression of GST and NQO1
and their mRNA transcripts; additionally, the liver and lung of mice treated with dietary curcumin had reduced oxidative stress.
The activated Nrf2 protein then binds antioxidant-responsive elements within the promoter regions of the antioxidant genes and induces transcription of a series of antioxidant enzymes, including NADPH quinone oxidoreductase (NQO1
), glutathione-S-transferase (GSH), heme oxygenase1 (HO-1), and [gamma]-glutamylcysteine synthetase [18, 21-23] (Figure 1).
Specific primers for heme oxygenase (decycling) 1 (HO-1), NAD(P)H dehydrogenase [quinone] 1 (NQO1
), glutamatecysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase regulatory subunit (GCLM), and nuclear factor (erythroid-derived 2)-like 2/NF E2-related factor (Nrf2) were designed, as well as primers for several housekeeping genes (see Supplementary Table 1 available online at https://doi.
In addition, cinnamaldehyde, an important flavor component in cinnamon essential oil upregulated Nrf2 expression, stimulated its translocation to the nucleus, and increased HO-1, NQO1
, CAT, and GPx1 expression under high glucose conditions .