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The 8 genes, which were chosen as the most over- or underexpressed, were EED, ARID1A, ING5, CTBP1, CBX3, CBX7, MTA1, and NSD1. Also, a housekeeping gene, HPRT1, was selected for RT-qPCR.
NSD1 mutation has recently been linked to the blocking of cellular differentiation and the promotion of oncogenesis in this type of tumor.
 Human genes: EGFR, epidermal growth factor receptor; KRAS, Kirsten rat sarcoma viral oncogene homolog; TP53, tumor protein p53; NF2, neurofibromin 2 (merlin); AKT1, v-akt murine thymoma viral oncogene homolog 1; BRAF, B-Raf proto-oncogene, serine/ threonine kinase; NRAS, neuroblastoma RAS viral (v-ras) oncogene homolog; KMT2D, lysine (K)-specific methyltransferase 2D; NSD1, nuclear receptor binding SET domain protein 1; CREB3L1, cAMP responsive element binding protein 3-like 1; TPR, translocated promoter region, nuclear basket protein; TSC2, tuberous sclerosis 2.
NSD1 Mitigates caspase-1 activation by listeriolysin O in macrophages.
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