tr[DELTA]SacI-1-GS115 and tr[DELTA]NsiI-1-GS115 strains carry PHO5 gene under the control of AOX1 gene promoters that were truncated using SacI and NsiI
Before the introduction of MS PCR, many of the patient samples had been tested by a published multiplex PCR combined with restriction enzyme digestion with AvaII and NsiI
described by Taube et al.
Genomic DNA (5-10 [micro]g) of cuphea-TE transgenic lines was digested with the restriction enzyme NsiI
, separated by electrophoresis on 0.8% (w/v) agarose gel, and blotted onto nylon hybridization transfer membrane Hybond-N+ (Amersham Pharmacia, Amersham, UK).
Herve Hamon and Patrick Rotman, Les Porteurs de valises: La Resistance francaise a la guerre d'Algerie (Paris: Albin Michel, 1979), Martin Evans, 'Memories of resistance to the Algerian war: Janine Cahen, Roger Rey, Denise Barrat', Modern and Contemporary France, NSii
, 2 (1994), 165-74.
DNAs were digested with the following restriction enzymes: AvaI, BamHI, BclI, BglII, CfoI, ClaI, DraI, EcoRV, HaeIII, HindIII, HpaI, KpnI, NcoI, NruI, NsiI
, PstI, PvuII, SacI, SalI, SmaI, XbaI, XhoI, XmnI.
The nucleotides underlined are mismatches with the CYP3A4 sequence, creating a NsiI restriction site in the wild-type CYP3A4 PCR product.
The PCR product (15 [micro]L) was digested with 10 U of NsiI (Roche) for 2 h at 37 [degrees]C, and analyzed on a 3% MP agarose/Tris-borate-EDTA gel with ethidium bromide staining.
For an initial assessment of rDNA variation in cotton, approximately 3 to 5 [micro]g of DNA from 16 accessions-cultivars (Table 1) was restriction digested with the enzymes BamHI, BgII BstEII, DraI, EcoRI, EcoR V, HindIII, KpnI, NcoI, NdeI, NsiI
, SacI, ScaI, SpeI, SspI, XbaI, and XhoI.