Mitochondrial DNA (mtDNA) cytochrome c oxidase subunit I gene (COI) has been successfully used for characterizing interspecific and intraspecific variability in different mollusks, in particular for arks shells in Asia (Cho et al., 2007; Chee et al., 2011a; Feng et al., 2011; Tanaka & Aranishi, 2013, 2014, 2016), and was thus applied to characterize Anadara tuberculosa population genetic structure within the NSMT.
Animals were collected from September 2010 to March 2011 by certified extractors within the area of the National Sanctuary Mangrove of Tumbes (NSMT), located from Boca de Capones and International Channel (3[degrees]18'S) to Playa Hermosa (3[degrees]35'S), and 80[degrees]13'08"W to 80[degrees]31'53"W (Zarumilla, Peru).
After collection from the NSMT, healthy Anadara tuberculosa brooders were brushed to eliminate attached material and placed in 1 L plastic box filled with filtered seawater.
From August 2010 to January 2011, 463 animals between 30 and 62 mm length were collected in the protected area of NSMT and kept in the hatchery as previously described.
Data were obtained from 111 animals collected in seven areas of the NSMT. All sequences obtained were processed by Blast (NCBI) and showed 99 to 100% homology with the A.
Tomokuni, 2[male] (00378726, 00378727), 29 (00378727, 00378728) (NSMT).
Six female specimens bearing the same label data as in males are present in the collection of NSMT. These females (Figs.
Tomokuni, light trap, 5[female] (00378945-00378949) (NSMT).
We thank several museum workers who provided access to specimens and loan of material for this study: Barry Hutchins, Gerry Allen and Sally Morrison (WAM), Jeff Johnson (QM), Helen Larson (NTM), Mark McGrouther (AMS), Keichii Matsuura (NSMT
), Sue Jewett and Jeff Williams (USNM) and Arnold Suzumoto (BPBM).