NSRBCNeuraminidase-treated Sheep Red Blood Cells
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The cells were incubated in humidified atmosphere containing 5% [CO.sub.2] for 18 hours at 37 [degrees] C After extensive washing with Hank's solution (GIBCO BRL), the PBMC were further purified by rosetting for 1 h at 4 [degrees] C with neuraminidase-treated sheep red blood cells (NSRBC), using a modification of a previously described method [19].