NTNHNon-Toxic Non-Hemagglutinin
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Liver and intestine samples from each bird were submitted for culture and to test for presence of the botulinum neurotoxin genes (BoNT) A and F as well as the nontoxic, nonhemagglutinin (NTNH) used as a surrogate marker for BoNT-producing clostridia.
Therefore, it was postulated that 19 S and 16 S toxins have both haemagglutinin (HA) and non-toxin non-haemagglutin (NTNH) proteins whereas 12 S toxin is formed by association of NTNH protein only.