Interestingly, stimulation of HT29 cells with NT resulted in ligand-induced internalization of NTR3/NT complexes suggesting that contribution of NTR3 might be more important than NTR I in the process of NT internalization (20).
Involvement of the neurotensin receptor subtype NTR3 in the growth effect of neuroicnsin on cancer cell lines.
The expression of neurotensin receptors, namely, NTR1, NTR2, and NTR3, was determined by real-time RT-PCR, Western blot, and immunocytochemistry analyses.
However, the expression of NTR3 slightly diminished by 0.2 [+ or -] 0.3(n = 3) fold, as shown in Figure 1(C).
Neurotensin receptor localization was measured in these cells and it was verified that NTR1 is localized at the cell membrane, in the cytoplasm and in the nucleus, while NTR3 is mainly found in the nucleus (Figure 2).
BJ cells expressed the NTR1 and NTR3; NTR1 was located at the cell membrane, cytoplasm, and nucleus while NTR3 was exclusively located in the nucleus.