and Pluripotency: A Complex Balancing Act," Cell Stem Cell, vol.
They pulled down approximately 100 proteins, including some members of the "NuRD
repressor complex" that is known for tightly winding up sections of DNA in a way that turns off a variety of different genes.
The functional consequences of mutations in CHD4, which encodes the catalytic subunit of the NuRD
chromatin-remodeling complex, remain to be elucidated; however, the designation of CHD4 as a significantly mutated gene in serous and serous-like tumors (15, 17, 18) and the presence of mutation hot spots within this gene strongly suggest it is likely to be a causal driver gene.
For example, PICKLE (PKL), a plant homologue of the animal chromatin remodeling factor CHROMODOMAIN/HELICASE/DNA-BINDING DOMAIN 3/Mi-2 (CHD3/Mi-2) which functions as a subunit of NUCLEOSOME REMODELING and DEACETYLASE (NuRD
) repressive complex as well as PICKLE RELATED 2 (PKR2) have trithomx-like functions, while CURLY LEAF (CLF) which is known to mediate the Irimethylation of H3K27 is a polycomb group protein.
Keeping things quiet: Roles of NuRD
and Sin3 co-repressor complexes during mammalian development.