2 mL of clotted blood and 1 mL of fresh EDTA anticoagulated blood were collected for serum alpha-1 AT assay and nitroblue tetrazolium
Laboratory evaluation for neutrophil's oxidative burst was performed and diagnosis of chronic granulomatous disease (CGD) was confirmed by both negative Nitroblue tetrazolium
test (NBT) and lack of dihydrorhodamine (DHR) fluorescence following neutrophil stimulation against phorbol myristate acetate (PMA).
Abbreviations CPZ: Chlorpromazine PS: Phosphatidylserine AchE: Acetylcholinesterase RBCs: Red blood cells PB3: Band 3 protein Hb: Hemoglobin GEs: Glycolytic enzymes G6P: Glucose-6-phosphate PPP: Pentose phosphate pathway HOS: High-oxygenation state LOS: Low-oxygenation state DNPH: 2,4-Dinitrophenylhydrazine p-NPP: p-Nitrophenylphosphate Met-Hb: Methemoglobin PMSF: Phenylmethanesulfonylfluoride NBT: Nitroblue tetrazolium
PMS: Phenazine methosulphate t-BHT: tert-Butyl-hydroperoxide EDTA: Ethylenediaminetetraacetic acid TBARS: Thiobarbituric acid reactive substances OV: Orthovanadate DNP: Dinitrophenyl FACS: Fluorescence-activated cell sorting SD: Standard deviation pHi: Cytoplasmic pH.
Fructosamine test was conducted using nitroblue tetrazolium
Immunologic studies including serum IgG IgA and IgM and phagocytic function tested with nitroblue tetrazolium
were within normal limits.
6-Hydroxy-2,5,7,8tetramethylchroman-2-carboxylic acid (Trolox), Ethylenediaminetetracetic acid (EDTA), Nitroblue tetrazolium
chloride (NBT) and Hydrogen peroxide ([H.
17 mM nitroblue tetrazolium
(NBT) salt in ethanol, 13 mM methionine, 0.
Para evaluar la respuesta inmune se utilizo el metodo de reduccion de Nitroblue Tetrazolium
(NBT) por ensayo espectrofotometrico descrito en Mohanty & Sahoo (41).
Superoxide production assay: Superoxide production of neutrophil was measured by nitroblue tetrazolium
(NBT) reduction assay following the method described by Nagahata et al.
A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitroblue tetrazolium
The present study evaluated the assay to quantify the respiratory burst activity of blood leukocytes of pacu as an indicator of the innate immune system, using the reduction of nitroblue tetrazolium
(NBT) to formazan as a measure of the production of reactive oxygen species (ROS).
14), (15) Among these, the colorometric assay (introduced in 1983), based on the ability of ketoamines to reduce nitroblue tetrazolium
(NBT assay) to produce a compound that is detected by absorption at 525 nm, has been adapted for many automated chemistry analyzers and is the most commonly used method.