The molecular diagnostic tests segment is further categorized into RT-PCR, nucleic acid sequence-based amplification
(NASBA) tests, loop-mediated isothermal amplification-based assays, and simple amplification-based assays (SAMBA).
 Nonstandard abbreviations: RBS, ribosome binding site; NASBA, nucleic acid sequence-based amplification
Different isothermal molecule amplification assays have been developed as a rapid, simple, and cost-effective alternative to PCR-based molecule assay since 1990s, which include loop-mediated isothermal amplification assay (LAMP) , helicase-dependent amplification assay , rolling circle amplification assay , nucleic acid sequence-based amplification
assay , transcription-mediated amplification assay , signal-mediated amplification assay , self-sustained sequence replication assay , ramification amplification assay , and RPA assay .
Quantitative real-time polymerase chain reaction and isothermal amplification techniques, including loop mediated isothermal amplification and nucleic acid sequence-based amplification
, represent some important improvements in the detection field.
Detection of dengue viral RNA using a nucleic acid sequence-based amplification
Individual paper topics include high throughput sequencing and comparative genomics of the foot-and-mouth disease virus, detecting virus using nucleic acid sequence-based amplification
, development of a framework for international certification of certain diagnostic tests, recent constraints on transport of materials, use of molecular diagnosis methods to prevent diseases in formed shrimp, SARS and avian coronaviruses, and antigen mass evaluation.
The nucleic acid sequence-based amplification
(NASBA) is a very sensitive and rapid technique for amplifying RNA and for detecting bacteria.
At the Provincial Laboratory, WNV RNA was detected in the plasma by nucleic acid sequence-based amplification
, with primers described by Lanciotti and Kerst (4), which was confirmed by the Artus RealArt RTPCR assay (arms biotech USA Inc, San Francisco, CA, USA) in a Roche LightCycler.
This simple test, which was developed in collaboration with the CDC in 2000, combines nucleic acid sequence-based amplification
(NASBA) technology with molecular beacon probe detection in the EasyQ System.
(10) There are currently 3 commercially available procedures for HCV viral load testing: the Roche (Belleville, NJ) AMPLICOR manual and COBAS HCV assays; the Bayer (Norwood, Mass) QUANTIPLEX HCV RNA version 3.0 assay (branched DNA [bDNA]); and the Biomerieux (Marcy-l'Etole, France) NucliSens system, which uses nucleic acid sequence-based amplification
The sensitivity of nucleic acid sequence-based amplification
compared to standard serologic testing in detecting cytomegalovirus (CMV) may allow earlier treatment of the infection in transplant patients.
Nucleic acid sequence-based amplification
. Nature 1991; 350:91-2.