Caption: FIGURE 4: A signaling network was constructed by IPA core analysis from the MetS-FL miR trio, the eleven common miR targets of MetS-FL miRs, ZO1, and OCLN
. Using Pathway Designer (IPA), miR-890 and miR-190 were added to the network based on present data (Figure 1) and literature .
Upon transmission, HCV enters hepatocytes in a multistep process involving the four host factors SR-BI, CD81, CLDN1, and OCLN
. After uptake and pH-dependent fusion of viral and endosomal membranes, HCV releases its RNA genome into the cytosol of the host cell.
Target Gene ID Primer Sequence ICAM1 25464 FP 5'-TGCAGGTGAACTGCTCTTCCTCTT-3' RP 5'-AGCTTCCAGTTGTGTCCACTCGAT-3' Tnf 24835 FP 5'-CCACCACGCTCTTCTGTCTACTG-3' RP 5'-GGGCTACGGGCTTGTCACTC-3' Il6 24498 FP 5'-TCCTACCCCAACTTCCAATGCTC-3' RP 5'-TTGGATGGTTCTTGGTCCAATGCTC-3' Il1b 24494 FP 5'-TGTCACTCATTGTGGCTGTGGAGA-3' RP 5'-TGGGAACATCACACACTAGCAGGT-3' Ocln
83497 FP 5'-TGGGACAGAGCCTATGGAACG-3' RP 5'-ATCACCAAGGAAGCGATGAAGC-3' Tjp1 292994 FP 5'-GATGAGCGGGCTACCTTATTGA-3' RP 5'-AGCGAACTGAATGGTCTGATGC-3' Cldn1 65129 FP 5'-TGCCCTACTTTCCTGCTCCTGT-3' RP 5'-AGTAGAAGGTGTTGGCTTGGGATAA-3' Cldn5 65131 FP 5'-CAGCGTTGGAAATTCTGGGTCT-3' RP 5'-CGTCTGCGCCGTCACGATAT-3' Actb 81822 FP 5'-CGCTCGTCGTCGACAACGG-3' RP 5'-TGTGGTGCCAAATCTTCTCC-3' FP: forward primer; RP: reverse primer.
CD81 along with human scavenger receptor SR-BI, and tight junction molecules claudin-1 (CLDN) and occludin (OCLN
) are the most important receptors that mediate HCV entry (46).
We were able to identify a differential expression of occludin (OCLN
), several claudins (CLDN 1-4, 7, 8, 12, 17-19, 22, and 23), and zonula occludens (ZO) tight junction proteins (ZO-1, ZO-2, and ZO-3) (Table 1).
M2 EQUITYBITES-June 6, 2016-OriginClear to trade under new ticker symbol OCLN
OriginClear Inc reported on Monday a change in its stock ticker symbol to 'OCLN' following approval from the Financial Industry Regulatory Authority (FINRA).