OD492optical density readings at 492 nm
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The optimum cutoff point of ELISA was determined by receiver operative curves (ROC) analysis using normalized OD492 value (PP%) as described elsewhere [12, 13].
The absorbance data (OD values) was normalized to eliminate interplate variations and make data comparable across the plates using the following formula: "percent positivity (PP%) = (OD492 sample serum--[OD.sub.492] negative control)/(OD492 positive control--[OD.sub.492] negative control) x 100%".