OD650Optical Density at 650 nm
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The colony concentration was calculated from the OD650 by the ATCC27853 standard curve (Y = 1.26514 x 10 - 8X + 0.48028, with a linear range between 1.5 x [10.sup.8] and 9.6 x [10.sup.3] CFU/mL, p < 0.01).
Finally, the peroxidase substrate was added and proliferation assessed by colorimetric detection (read at OD650) using a microplate reader (1420 [Vitor.sup.3], Perkin-Elmer).
The optical density at 650 (OD650) was measured using a precision microplate reader (Molecular Devices.