OD650 - What does OD650 stand for? The Free Dictionary
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The colony concentration was calculated from the OD650
by the ATCC27853 standard curve (Y = 1.26514 x 10 - 8X + 0.48028, with a linear range between 1.5 x [10.sup.8] and 9.6 x [10.sup.3] CFU/mL, p < 0.01).
Finally, the peroxidase substrate was added and proliferation assessed by colorimetric detection (read at OD650
) using a microplate reader (1420 [Vitor.sup.3], Perkin-Elmer).
The optical density at 650 (OD650
) was measured using a precision microplate reader (Molecular Devices.