Cell viability was assessed by the measurement of the reduction of 3-(4, 5-dimethyIthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) to produce a dark blue formazan product (Hansen and Nielson, 1989), MTT was added to each well after OGD
injury at a final concentration of 0.5mg/ml.
Implementing that goal, combined with an increased number of applications, has made it difficult for the OGD
to respond to sponsors or non-review related issues.
At the FDA Center for Drug Evaluation and Research's (CDER's) OPS and OGD
, Tan was the recipient of multiple awards for operations leadership and technical expertise in bioequivalence and paragraph IV filings.
bEnd.3 cells (2 x 105 cells/mL) were seeded in 98-well plates to monitor all experiment conditions, including pretreatment, OGD
injury, and reperfusion.
Besides that reorganization, OGD
is preparing to move its personnel and resources to White Oak, Md., where the FDA maintains its main campus.
40% of PC12 cells were killed by OGD
for 8 h and reperfusion for 24 h relative to a sham control.
A gas gathering network from Thamama C and F linking up with OGD
was completed in 1996.
Like his industry peers, Bisaro called for the need for continued pressure on policy makers to provide sufficient funding to the OGD
, particularly as the debate over user fees is renewed.
To increase the sulphur handling capacity for its OGD
system, called Project 1205 and costing $15m, ADNOC awarded the contract in 1994 for completion before end-1994.
"We will continue to encourage Congress to strengthen this investment in the OGD
," comments Jaeger.
We will have a host of issues on our plate, including the continued battle over biogenerics legislation, drug safety and foreign inspections, funding for the Food and Drug Administration's office of generic drugs (OGD
), state carve-out legislation, and bioequivalence.
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