5 [micro]g/mL administered dose) was observed in ORF1 and ORF2
, although it was not statistically significant (p-value 0.
The region amplified was at the 3'-most end of ORF2
Las microplacas de los estuches utilizados en el presente estudio, estaban recubiertas con antigenos sinteticos especificos de HEV derivados de las regiones ORF2
y ORF3 de los 4 genotipos segun lo descrito por el fabricante.
Foram identificados seis dominios antigenicos na ORF2
e tres na ORF3 (99).
Database analysis revealed that the primary structure of protein encoded in ORF1 and ORF2
showed high similarity with aldehyde dehydrogenase and iron containing alcohol dehydrogenase of P.
Considering that the inespecifity of primers which amplify the UTR, and that for some animals species the UTR region is located close to the ORF2
region, two samples (one of Cebus apella and one of Callithrix jacchus) were amplified only with ORF primers.
Assays for HEV antibodies should detect antibodies to the ORF2
antigen to assure adequate clinical specificity (IIIB and IIIE).
Under the terms of the agreement, SmithKline Beecham was granted a non-exclusive license to the recombinant Hepatitis E virus ORF2
capsid genes cloned into baculoviruses for commercial development, manufacturing, and marketing of Hepatitis E virus vaccines.
Separately, ORF1 failed to reach the required level, but ORF2
was well over it.
ORF1 encodes a 40-kD RNA-binding protein, and ORF2
encodes a protein with endonuclease and reverse transcriptase (RT) activities.
In the field of television, the Germans have put a lot of products on the market because they allow private ownership, while Austria has made do with the government-owned ORF1 and ORF2
Yet, ORF1 and ORF2
are more than holding up their end in the face of all that competition.