ORF6Open Reading Frame 6
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With the advent of PCR, it was feasible to use in situ hybridization to diagnosis PRRSV by detecting expression using probes for ORF1b, ORF6, and ORF7 (Pirzadeh and Dea, 1998; Kwang et al., 1999).
Gene ID of viral genes (nucleotide base numbers for complete genome) FMD serotypes Type O Type A VP1 HM143846.1 GU441855.1 2B NC_004004.1 (3918-4379) KC588943.1 (3950-4411) 3D KF1 12888.1 (7145-7880) KC588943.1 (7148-7883) PRRS serotypes North America 1 North America 2 ORF5 DQ473474.1 (13788-14390) KF555450.1 (13395-13997) ORF6 DQ473474.1 (14375-14899) KF555450.1 (13982-14506) ORF7 DQ473474.1 (14889-15260) KF555450.1 (14496-14867) FMD serotypes Type Asia 1 VP1 DQ121129.1 2B JF739177.1 (3947-4408) 3D JF739177.1 (7145-7880) PRRS serotypes Korean ORF5 AY585241.1 (13788-14390) ORF6 AY585241.1 (14375-14899) ORF7 AY585241.1 (14889-15260) ORF, open reading frame.
Further studies by Mulligan showed that this glycoprotein was a previously undescribed mammalian protein, sharing significant sequence homology with the gene product of a micro-organism (ORF6) implicated in cell adhesion.
The genome of PRRSV is 15 kb in length and contains nine open reading frames (ORFs) designated ORF1a, ORF1b, ORF2a, ORF2b,ORF3, ORF4, ORF5, ORF6, and ORF7 [21-23].
Roles for the E4 orf6, orf3, and E1B 55-kilodalton proteins in cell cycle-independent adenovirus replication.