In this case, the team set up a library of about 100 million distinct antibodies and used it to find any that could substitute for OSKM
Buganim hypothesizes that SNEL may reprogram cells better than OSKM
because it does not rely on the master regulators Oct4 and Sox2, which might activate part of the adult cell genome.
Buganim hypothesizes that SNEL reprograms cells better because, unlike OSKM
, the cocktail does not rely on a potent oncogene like Myc, which may be causing some of the genetic problems.
The challenge with the previous OSKM method has been that very few cells actually become iPS cells during reprogramming.
The efficiency of making mouse and human iPS cells was increased over 50-fold compared with the standard OSKM combination.