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Related to OSMIC: osmic acid
OSMICOpen Systems Management Industry Collaboration (Japan)
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Sections of white matter from the spinal cord of the control (A) and sham (B) groups, as seen by staining with osmic acid, revealing darkly stained intact myelin sheaths; seven days after CSCI, nerve fibers were lost, and the number of myelinated nerve fibers appeared to be minimal (C); scale bars A-C, 50 mm.
The cell-scaffold constructs were fixed with 2.5% glutaraldehyde and then osmic acid, dehydrated in a graded ethanol series and dried by critical-point drying, and then sputter-coated with goldpalladium before observation.
After being washed three times with 0.01 M phosphate buffer, samples were post-fixed in 1% osmic acid for 3 hours at 4[degrees]C.
We abstract the above BT 325 cells which have been treated by matrine and fix them by 1% osmic acid after washing and then wash them with 0.1% PBS.
Moreover, the piece was fixed in 1% osmic acid again at 4[degrees]C for 1.5 h after being rinsed with phosphate buffer.
Lung tissue samples were removed from fixed specimens fixed by 2.5% glutaraldehyde phosphate buffer and then by 1% osmic acid fixation, gradient acetone dehydration, Epon812 embedded agent, and ultrathin sections (thickness 60 nm), as uranium and lead citrate double staining, then observed under Hitachi H-7500 transmission electron microscope and photographed with Germany CCD camera system.
3 mm nerve segment in the middle part of nerve graft and the parallel segment in the control nerve were taken to fix in 4% glutaraldehyde in PBS for 4hat room temperature, then rinsed in PBS 3 x 3 min, fixed in osmic acid, rinsed in PBS 3 x 3 min, and dehydrated in ascending series of alcohol (50%, 70%, 90%, and 100%); Epon812 embedded, semithin section of 1 [micro]m was toluidine blue stained.
The liver samples were washed three times for 10 min each with sodium dimethyl arsenic acid buffer (pH 7.2) and fixed in 1% osmic acid for 2 hr at 4[degrees]C.
In their attempt to quantify the effect that solar activity-whether directly or through osmic rays-may have had on global temperatures in the twentieth century, Sloan and Wolfendale compared data on the rate of cosmic rays entering the atmosphere, which can be used as a proxy for solar activity, with the record of global temperatures going back to 1955.