The modal number of chromosomes differed in Owt (66), Owti (69), and Om (70/71) cybrids; however, these numbers are similar to those previously published in other osteosarcoma 143B transmitochondrial cell lines (Gomez-Duran et al.
In contrast with the Om cybrids, in which [H.sub.2][O.sub.2] levels were significantly higher than in Owti, [H.sub.2][O.sub.2] levels were decreased in Am cybrids compared with Awt, albeit not significantly so.
Furthermore, Owti and Awt cybrids treated with TBTC [greater than or equal to] 60 nM and TBTC [greater than or equal to] 90 nM, respectively, showed significantly reduced ATP levels.
We found that > 30 nM TBTC significantly decreased [H.sub.2][O.sub.2] production in Owti and Om cybrids when compared with untreated cybrids (Figure 4).
At TBTC concentrations [greater than or equal to] 20 nM, Owti IDH activity did not differ significantly from that of untreated Owti cells.
Import tariffs as percentage of imports (Tariffs), tariffs on intermediate inputs and capital goods (
Owti), and total import charges (Totimpov) can all be considered as good proxies of trade restrictiveness and have also been employed in this study.