References in periodicals archive ?
PBAE polymers A1 (bis(3-(propionyloxy)propyl)3,37-(propane-1,3-diyl-bis(methylazanediyl))dipropanoate) and A2 (bis(4-(propionyloxy)butyl)3,3,-(ethane-1,2-diyl-bis(isopropylazanediyl))dipropanoate) were synthesized in our lab.
Microparticles of PLGA and PBAE polymer blend were prepared at different ratios, namely, 85: 15, 75: 25, 50: 50 using dichloromethane (DCM) as solvent.
Microparticles, comprising a blend of PBAE and PLGA polymers, were prepared by DESR method.
With increase in PBAE content above 15%, the microparticles exhibited delayed release.
With increase in PBAE content, there was a slight decrease in cell viability.
Among the polymer blend microparticles, with increase in PBAE content, the transfection efficiency was decreased.
In our present study, we have used novel PBAE polymers synthesized in our lab.
Using DESR method, the microparticles were successfully prepared with blend of PLGA and PBAE at different ratios (85: 15, 75: 25, 50: 50) with size range of 0.8 to 1.9 [micro]m which was suitable for passive deliveryinto phagocytic cells, a prime need for DNA vaccine.
The proportionate increase in encapsulation efficiency with increasing PBAE content in polymer blend microparticles may be correlated to the electrostatic interactions between pDNA and PBAE .
The rough surface observed with increase in PBAE content could be due to the difference in rate of evaporation of the common solvent from the polymers [27, 28].
However, the sustained release pattern observed with increased PBAE content could be because of binding of free PBAE with free DNA, which could have masked the detection of DNA or could have resulted in sustained release of DNA over time.
Acronyms browser ?
Full browser ?
- Pb-I-Pb junction