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The diet of the animals consisted of 50% of corn silage and 50% of commercial concentrate (Table 1), containing two treatments with propolis-based products at different dosages (PBP1 and PBP2) and a control treatment without addition of additives (CON).
The PBP1 and PBP2 products were added to the feed at the time of feeding.
The CON treatment had greater weight gain (1.98 kg [day.sup.-1]), but did not differ from treatment with the highest dosage of propolis (PBP2), with an average of 1.85 kg [day.sup.-1]; for the PBP1 treatment, it was observed the lowest weight gain during this period.
The addition of PBP1 reduced the fermentation of cellulose when expressed as a percentage of tolerant bacterial strains, but this effect was lower in diets based on forage.
(2010b) for diets containing the same forage:concentrate ratio (50:50), who observed an increase from 8.3% and 6.2% in vitro DM digestibility with the addition of PBP1 (p < 0.05) compared to the control and to monensin, respectively.
For example, in group G1 isolates, the percentage of densitometric values of PBPs ranged between 30-63 per cent (PBP1); 33-63 per cent (PBP2); 68-83 per cent (PBP3); 11-91 per cent (PBP4); 66-16 per cent (PBP5); 86-98 per cent (PBP6); 49-69 per cent (PBP1); 95-99 per cent (PBP8) with reference to the respective PBP expression in ATCC.
The probability of being in or exceeding a damage state for typologies PB1, PB2, PBP1 and PBP2 are given in Figures 6, 7, 8, and 9.
The ponA gene, which encodes PBP1, had an amino acid substitution at position L421 (L [right arrow] P), and full-length PilQ amino acid sequence had sequence type VI.
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