References in periodicals archive ?
The plates were washed three times with PBST at each step.
The plate was washed 3 times with PBST and blocked with 3% skim milk-PBST for 1 hr at 37[degrees]C.
The plates were washed with PBST. Different dilutions of EPEC cells ([10.sup.9]-[10.sup.0]) were added to the wells followed by incubation at 37 [degrees]C for an hour.
Control larvae were incubated overnight in PBST without primary antibody.
 Nonstandard abbreviations: AD, Alzheimer disease; CSF, cerebrospinal fluid; WashU, Washington University; ADx, ADx Neuro Sciences; UGot, University of Gothenburg; DLB, dementia with Lewy bodies; FTD, frontotemporal dementia; VaD, Vascular dementia; PBST, PBS containing 0.05%Tween-20; LLOQ, lower limit of quantification; ANCOVA, analysis of covariance; MMSE, Mini-Mental State Examination.
The NCM was blocked with 3% bovine albumin for 1 h at 37[degrees]C and washed three times for 5 min each with PBST. The NCM was then incubated separately in four PcAbs for 1 h at 37[degrees]C, followed by three washings with PBST.
Slides were then dipped in blocking and permeabilization buffer (PBST with 5% BSA and 0.5% Triton X-100) for 30 min.
The plates were then washed with 0.2% Tween-20 in PBS (PBST) for three times, and serial twofold dilutions of the sera in PBS, starting at an initial dilution of 1: 200 to a final dilution of 1: 2,04,800, were added to the plates (100 [micro]L/well).
Each well was then washed 3 times with 200 [micro]l of 1X PBST and blocked with 150 [micro]l of SuperBlock T20 (Thermo Fisher Scientific, Waltham, MA, USA) for 1 h at RT on a shaker.
To block nonspecific binding, the membrane was incubated with 5% bovine serum albumin (BSA) in PBST (0.1% Tween-20 in PBS) for 1h at room temperature.
Plates were washed with PBS-0.1% Tween (PBST), incubated for 2 h at 37[degrees]C with blocking solution (PBS containing 5% skim milk), washed with PBST and PBS, and then incubated with 50 f L of either mouse anti-rTcENO or anti-pBKTcENO (1 : 500 dilutions).
A solution of 0.3% Triton-X 100 in PBS (PBST) is used in a permeabilization step to open up the cell membrane and allow for the antibodies to enter.
Acronyms browser ?
Full browser ?