PCPV

AcronymDefinition
PCPVProfessional Certificate in Pharmacovigilance (India)
PCPVProyecto ContraSida Por Vida (defunct homeless shelter; San Francisco, CA)
PCPVPrestressed Concrete Pressure Vessel
PCPVPrograma Crer Para Ver (Portuguese: Believe to See Program)
References in periodicals archive ?
Embora as lesoes causadas pelo PCPV e BPSV sejam confundiveis com febre aftosa, estomatite vesicular e vaccinia bovina (RIET-CORREA et al., 1996; SANT'ANA et al., 2012), pouca importancia tem sido dada a esses agentes.
Apos o resultado negativo nos testes para esses virus, suabes das lesoes, fragmentos de tecidos e crostas foram enviados para a pesquisa de agentes que fazem parte do diagnostico diferencial destas enfermidades, como o VACV, BPSV, PCPV e herpesvirus bovino do tipo 2 (BoHV-2).
O sequenciamento de nucleotideos dos amplicons revelou que tres propriedades possuiam animais infectados pelo PCPV (97-99% de identidade) e, em uma propriedade o virus identificado foi o BPSV (97-99% de identidade) (Figura 2).
O PCPV foi identificado em lesoes de bezerros da propriedade SV721/12, o que pode indicar que os animais da propriedade SV719/12 tambem fossem positivos para esse virus, embora o PCR tenha sido negativo.
A identificacao dos parapoxvirus PCPV e BPSV nesse surto ressalta a importancia de se investigar esses agentes virais em casos de doenca vesicular de bovinos.
As lesoes causadas pelos parapoxvirus PCPV e BPSV sao indistinguiveis clinicamente, e cursam com lesoes vesiculo-pustulares que, eventualmente, evoluem para crostas, geralmente nos tetos, focinho e cavidade oral de bovinos (SCHATZMAYR et al., 2000; BUTTNER & RZIHA, 2002; FLEMMING & MERCER, 2007).
The PPV real-time PCR assay was designed as a consensus PCR to specifically amplify DNA from all PPV species: ORFV, PCPV, BPSV, and SPV.
We assessed the performance of the PPV real-time PCR assay with, as calibrators, 4 plasmids that were cloned according to routine procedures and contained 487 by of the B2L gene of ORFV (strain B006, pORFV), PCPV (strain B021, pPCPV), BPSV (strain B177, pBPSV), and SPV (pSPV), including the original sequence of these isolates in the primer-binding (PPV up, PPV do) and probebinding (PPV TMGB) region (cloning primers are shown in supplemental material Table 1 in the online Data Supplement).
Although there is a divergence of up to 15.6% in the B2L gene sequences of different PPV species, careful selection of primers and probe gave satisfactory and comparable amplification efficiency as well as a detection limit for the PPV species ORFV, BPSV, PCPV, and SPV, which was confirmed by analysis of 41 clinical samples from humans or animals infected with these PPV species.
Molecular methods allowed for the detection and characterization of VACV (3 patients) and PCPV (2 patients).
Analysis and characterization of VACV and PCPV complete genomes could provide clues to explain their emergence and recent evolutionary histories, and research aimed at identifying the domestic animal hosts and wildlife reservoirs of poxviruses could further our understanding of their natural transmission cycles.
Given the genetic similarity between the PPVs that are infecting red deer and cattle, ecologic studies should be designed to evaluate the susceptibility of these animal species, respectively, to PCPV, BPSV, and PVNZ.