PCR-RFLPPolymerase Chain Reaction Restriction Fragment Length Polymorphism (gene identification method)
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A PCR-RFLP of the groEL gene strategy, standardized by our group to differentiate several relevant Staphylococcus species (SANTOS et al., 2008), was then performed.
At present, molecular techniques such as PCR-RFLP and PCR-FP allow the identification of eight molecular types, associated with these species complex: VNI and VNII corresponding to C.
Validation of genotypes by PCR-RFLP were confirmed by DNA sequencing (Applied BiosystemsA(r) 3130 Genetic Analyser by Life Technologies; USA) done on samples selected randomly (Figure-2).
The molecular method based on rDNA-ITS region and PCR-RFLP has been an efficient approach to reveal phylogenetic relationship and molecular characteristics amongcyst nematode species.
The liquid based sample was used for an in-house HPV genotyping testing (PCR-RFLP analysis) and PapilloCheck[R] microarray.
In the present study, a PCR-RFLP method using universal COI primers was developed for the identification of six common nassarid species, including Nassarius nodifer, Nassarius conoidalis, Nassarius sinarus, Nassarius succinctus, Nassarius variciferus, and Reticunassa festiva, along the coasts of China.
The aim of this study was to determine genetic variation of calpastatin gene for G>A and C>T SNPs in Kivircik lambs by PCR-RFLP method with using MspI and NcoI restriction enzymes.
PCR and PCR-RFLP. A region of approximately 680bp of the ITS1 of rDNA in 96 samples was successfully amplified (Figure 2) as predicted.
In this study, a new pair of universal primers was designed and a species identification assay has been developed using the variable size fragments, and this assay can be further developed into PCR-RFLP assay, which showed potential as a tool for cost-effective, rapid, specific, and sensitive detection of goat, sheep, buffalo, cattle, yak, deer, pig, and camel simultaneously.
SNP A-1575G in promoter region and SNP A2497C in Intron1 could be detected by Taq I and HaeIII PCR-RFLP, respectively, and these SNPs wer selected for further association analysis.
For PCR-RFLP analysis, the PCR product was digested with Fok-I restriction enzyme.
For the gastric cancer and PCR-RFLP subgroups, no significant associations were found in any of the compared genetic models.