PCR-SSCPPolymerase Chain Reaction–Single Strand Conformation Polymorphism
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We detected polymorphisms within the mtDNA D-loop variable region through PCR-SSCP and found 12 haplotypes in 4 different types of local Mongolian horses and one captive breed.
The polymorphisms of the PCR products in all individuals were analyzed by PCR-SSCP. However, only the products of primers E4 exhibited polymorphism.
PCR-SSCP analysis on partial sequence of gonadotropin releasing hormone receptor gene in sheep.
The electrophoresis pattern of PCR-SSCP technique for MIR-196a2 gene, (Pattern A:3 bands) and (Pattern B:one band)for both patients and control
PCR-SSCP is a simple, sensitive and efficient technique for identifying the genetic variations in candidate genes.
Schmidt, "Application of PCR-SSCP to species identification of fishery products," Journal of the Science of Food and Agriculture, vol.
Group Gender Age Number of samples used HC Male 68.45 [+ or -] 1.282 20 PD Male 65.85 [+ or -] 1.19 20 HC Female 65.8 [+ or -] 1.133 10 PD Female 65.7 [+ or -] 1.202 10 Group FOXA1 mRNA mean + SME p value HC 1.373 [+ or -] 0.77 <0.05 PD 0.4884 [+ or -] 0.045 HC 1.681 [+ or -] 0.201 <0.05 PD 0.7436 [+ or -] 0.07 TABLE 5: Mean values ([+ or -]SEM) of mRNA expression ([2.sup.- [DELTA]ct]) with PCR-SSCP patterns of NURR1 and FOXA1 gene.
Sheep CAST gene fragment identified by using PCR-SSCP (single strand conformational polymorphism) methods.
Several assays are available to detect k-ras mutation, such as PCR-ASO-DBH, PCR-RFLP, hybridization protection assay, PCR-SSCP, and PCR-denaturing gradient gel electrophoresis.
Diverse genotyping methods were used, including polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) [23, 27], TaqMan [21, 24, 28], polymerase chain reaction-sequence specific primers (PCR-SSCP) [15, 25], MassARRAY [20, 22], and SNaPshot SNP assay [26] methods in eligible publications.
Materials and Methods: Polyrnerase chain reaction and single-strand conformation polymorphism (PCR-SSCP) were used to screen 55 AML patients for mutations of NPM1 gene.