PCR-SSO

AcronymDefinition
PCR-SSOPolymerase Chain Reaction-Sequence Specific Oligonucleotide Probe
References in periodicals archive ?
Comparison of HLA-DRBl typing by DNA-RFLP, PCR-SSO and PCR-SSP methods and their application in providing matched unrelated donors for bone marrow transplantation.
The method is relatively high-throughput and inexpensive compared to PCR-SSO and PCR-SBT, and enables highly accurate HLA typing by producing hundreds of base pairs of long sequence reads at high coverage depth.
PCR-SSO typing (One Lambda LABType[R] using Luminex[TM]): biotinylated primers were used.
Discrepant HLA typing results were detected in 6 of 33 samples (18.2%) analyzed by two different PCR-SSO techniques and one PCR-SSP technique.
The first sample was typed as HLA-A*24, A*30 by PCR-SSP and as HLA-A*24, A*32 by PCR-SSO. The second sample was typed as HLAA*03, A*30 by PCR-SSP and as HLA-A*02, A*30 by PCR-SSO.
The same sample was typed as HLA B*27, B*27 by PCR-SSP and as HLA-B*27, B*51 by PCR-SSO. The second sample was typed as HLA-B* 07, B*45 by PCR-SSP and as HLA-B* 07, B*50 by PCR-SSO.
The purpose of this consultation is to provide reagents for hla genotyping of the hla-a, -b, -c, -drb1, -dqb1, -dpb1, dpa1 and dqa1 loci by pcr-sso technique with intermediate and compatible resolution.
The purpose of this consultation is to provide reagents for hla genotyping of hla-a, -b, -c, -drb1, -dqb1, -dpb1, dpa1 and dqa1 loci by pcr-sso technique with intermediate and compatible resolution.
The subject of performance of this contract will be screening and identification of anti-HLA and anti-MICA antibodies genotyping HLA system using PCR-SSO, using techniques xMAP associated with the borrowing analyzer (based on flow cytometry using microsphere particles either with the bound HLA molecules or DNA probes) for Blood Centre - HLA / DNA laboratory of the University Hospital Ostrava.estimated value excluding VAT: 3 759 428.20 CZK
Supply with the form of service of instrumentation and reagents for molecular typing of HLA alleles with PCR-SSO and research anti-HLA-based Luminex technology for the needs of the Laboratory of Immunogenetics and Transplant Immunology - months validity 36 (thirty).
4) Part 4 - Gradual supply of test kits, reagents, Taq DNA polymerase, consumables for the determination of HLA class II antigens (DQB1) at low resolution PCR-SSO owned Luminex analyzer 100 / 2001S, reagents compatible with existing manual Lifecodes locus A , B, C, DRB1 in terms of technique and analysis of the signs of these assays, the amount necessary to carry 2 000 marks;
Supply of reagents needed for HLA typing class I and II through PCR-SSO technique on compatible equipment fluorometric continuous flow Luminex HLA types used in the laboratories of the EFS.