PCRRFLPPolymerase Chain Reaction Followed by Restriction Fragment Length Polymorphism Assay
References in periodicals archive ?
Polymorphisms rs2227501 (A/T), rs2227503 (A/G), rs1026786 (A/G) from IL-22 and rs4711998 (A/G) from IL-17 were selected and determined by polymerase chain reaction-restriction fragment length polymorphisms (PCRRFLP).
One of the most extensively studied milk protein polymorphisms is the substitution of the amino acid Tyr20 with His in the LGB polypeptide (6) resulting in a saI restriction fragment length polymorphism which permits the genotyping of animals with a PCRRFLP method (7).
Genotyping of CYP11B2 promoter (-344C>T, rs1799998) polymorphism: The CYP11B2 promoter polymorphism (-344C>T) was detected by PCRRFLP method as described elsewhere (16).
Among several methods, P32 gene based PCRRFLP is simple, specific, cheap and quick to differentiate SPPV and GTPV (Heine et al., 1999, Bhanuprakash et al., 2006, 2010).
Beauveria bassiana haplotype determination based on nuclear rDNA internal transcribed spacer PCRRFLP. Mycol.
Therefore, this study was aimed at detection of pork or derivative porcine contamination in commercial product of meatballs from Surabaya and Yogyakarta region of Indonesia using PCRRFLP methods through cytochrome b gene.
Matsumoto, "S-RNase genotypes of apple cultivars grown in Japan and development of A PCRRFLP method to identify the S6-and S21-RNase alleles," Journal of Horticultural Science and Biotechnology, vol.
This work was coupled with the ex vivo assessment of parasite responses to a panel of antimalarial drugs and genotyping for Pfcrt-K76T by PCRRFLP. Consistent with previous reports, similar trends were observed in vitro with artemisinin derivatives and other partner molecules, and also, more surprisingly, with CQ and AQ, with 93% and 96% of isolates responding adequately in vitro to these drugs, respectively.
La identificacion de la especie se realizo mediante la tecnica de los disenos perineales (Franklin 1962) y estudios moleculares mediante la tecnica de la Reaccion en Cadena de la Polimerasa (PCR) y analisis de PCRRFLP del ADN mitocondrial.
The other molecular techniques including; PCR and PCRRFLP on microsatellite loci and, Ace.2, COI, ITS genes were studied for the separation of these complex species.
La busqueda de la mutacion p.Q829X se realizo mediante la tecnica de PCRRFLP (Restriction FragmentLength Polymorphism).