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Sixty-five clinical samples (spleen, inguinal lymph node, tonsil, lung, and serum) were collected from suspected PCV2 infection pigs from eight pig farms in Shandong Province (China), thirty-seven clinical samples (inguinal lymph node, tonsil, lung, and serum) were collected from Gansu Province (China), and ten PCV1 positive samples were conserved in our laboratory, confirmed by real-time qPCR  and Sanger dideoxy sequencing.
Real-time qPCR assay for detection of PCV2 DNA and PCV1 DNA were both performed on Agilent Mx3005P thermocycler machine (Life Technologies, USA) as previously described [20, 21].
Their genomes consist of single-stranded DNA that is circular in nature with about 1759 (PCV1) and 1767-1768 (PCV2) nucleotides sequence [12, 44].
Caption: Figure 1: Genome organization of Porcine circovirus (NB: map (a) is for PCV1, while maps (b), (c), and (d) are for PCV2a and 2e; PCV2b and PCV2c and 2d, resp.), adapted from Zhai et al.
TABLA II PATOGENOS ENCONTRADOS EN TRES GRANJAS DEL CENTRO OCCIDENTE DE COLOMBIA EN LECHOS DE SECADO DE PORCINAZA Patogeno GG GM M1 M2 M1 M2 DI P DI P DI P DI P PVP PCV1 PCV2 PRRS HVP1 Lawsonia intracellularis + Actinobacillus pleuropneumoniae Mesofilos aerobios + + + + + Staphylococcus aureus + + + + Mohos + + Levaduras + + + + Clostridium sulfito reductores + + + + + + Coliformes totales + + + + + Escherichia coli + + + + + + + + Listeria monocytogenes Salmonella spp.
Now, the first stage bypass (PCV1) will be able to control main suction pressure (PT1) with PCV2 in parallel.
At that meeting, the FDA announced that Merck had recently notified the agency that PCV1 and PCV2 had been detected in that company's rotavirus vaccine, RotaTeq.
At the meeting, GSK presented results of studies conducted since PCV DNA was detected in Rotarix, which has not provided any evidence that PCV1 in Rotarix causes infections in humans, according to the presenters.
(18) de PCV1 y PCV2 en muestras de calostro de cerdas sugirio que la transmision del virus via oral a traves de la leche podria tambien ser posible.
has reported that the concentration of PCV-2 DNA fragment in clinical consistency lots was in the range of below limit of detection to 6.4 x [10.sup.3] copies/mL when measured by QPCR, and that PCV1 was below limit of detection (0.1-0.8 x [10.sup.3] copies/mL) .
"There is no evidence at this time that this material from PCV1 in Rotarix poses any safety risk," Dr.
PCV1 is a virus that is often found in meat and other foods, and it is not known to cause disease in humans or animals.
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