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Hence, pelB leader peptides were used to allow translocation of unfolded recombinant protein to the oxidizing space of periplasm.
The amount of positive charge in n-region was +2 in most signal peptides, while it was +1 in PelB signal peptide, +3 in LivK, MalE, and Pac signal peptides, and +4 in Endoxylanase signal peptide.
coli, like OmpA (13,14), PhoA (15), pelB (14,16), LTB (17), npr (18), StII (19,20), DsbA (8), penicillinase (21) and natural hGH signal peptide (22).
pelB and His--Tag coding sequences were introduced to the N-terminal and C-terminal of the construct respectively.