The mRNA abundance of hepatic PEPCK
, PC, CPT1, and PPARa, both prepartum and postpartum, was not affected by dietary energy density (p>0.05; Table 5).
Pyruvate then proceeds to phosphoenolpyruvate by the enzymes pyruvate carboxylase and PEPCK
The supernatant was separated and divided into two parts for measurement of phosphoenolpyruvate carboxykinase (PEPCK
) and glucose 6-phosphatase (G6Pase) activities.
(f) The gene expression levels of PEPCK
and G6Pase related to gluconeogenesis were determined by qPCR in liver tissues.
Rabbit anti-cytosolic PEPCK
was prepared in our laboratory .
Significantly upregulated proteins include glycerol-3-phosphate dehydrogenase (GPDH), PEPCK
, perilipins 1 and 4 (PLIN1/4), fatty acid binding protein 4 (FABP4), and adiponectin, all downstream targets of PPAR[gamma] (Figure 2E).
Marein affects glycogen synthesis and the expression of GSK-3[beta], FoxO1, G6Pase and PEPCK
in HepG2 cells
Phosphoenol pyruvate carboxykinase (PEPCK
), a key enzyme of gluconeogenesis in the liver, was upregulated significantly (p < 0.05) in diabetic rats compared with control group.
Liver, skeletal muscle and adipose tissue were also collected for mRNA gene expressions (GLUT-4, IRS-1, IR, PEPCK
, SREBP-1c, FAS, PPAR-a, PPAR-g, TNF-a).
(2007) showed that salt stress strongly enhances the activity of phosphoenolpyruvate carboxylase kinase (PEPCK
), which is known to be controlled by ABA in sorghum leaves.
(18) HWJMSCs population has been found to express the early hepatic markers, such as albumin, alpha-fetoprotein (AFP), cytokeratin-19 (CK-19), some mid- and late- hepatic markers, including CK18 and glucose 6-phosphatase (G6P), (19,20) as well as a low level of hematopoietic markers, such as c-kit and phosphoenolpyruvate carboxykinase (PEPCK
Chronic hyperglycemia enhances PEPCK
gene expression and hepatocellular glucose production via elevated liver activating and inhibitory protein ratio.