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We have also found phosphofructokinase (PFK) activity on the calcium regulatory vesicles isolated from native prophase mitotic apparatus.
To extend the hypothesis that an integrated metabolic network is involved in production of [LtB.sub.4], isolated endomembrane subfractions from prophase mitotic apparatus were assayed for phospho-fructokinase (PFK) activity.
In addition, we have found that PFK activity is part of a network of enzymes on perinuclear vesicles that regulate production of [LtB.sub.4] as an agonist of the discrete pre-NEB calcium signal (5, 12).