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The state Commandant, Mr Makinde Ayinla, disclosed this on Thursday in Benin, at a one-day seminar organised for Directors and Managers of PGCs.
After the PGCs have been specified as the independent germ lineage, they must make their way to the gonadal ridge, proliferating en route, where they will receive subsequent differentiation cues for sex-specific development.
Furthermore, the genetic modification of PGCs (via TALEN or CRISPR/CAS9) is the most efficient way to create genetically engineered bird models for basic and applied purposes [6, 7].
This metabolic shift, mitochondrial inactivation, and ROS clearance have also been observed in our hiEGL cultures when compared with nonreprogrammed PGCs .
The pGCs were aspirated from follicles 1 mm to 5 mm in diameter and placed into a sterile centrifuge tube containing 0.9% sodium chloride solution and centrifuged at 1500 x g for 5 minutes.
Thirty-six IVF hatched blastocysts on days 10-11 of IVC were randomly distributed into two 4-well plates (Nunc, Thermo Scientific, Denmark), grouped into MEF and PGCs groups, eighteen embryos each and three embryos per each replicate.
Researchers at the Gurdon Institute found that a gene known as SOX17 is critical for directing human stem cells to become PGCs (a stage known as 'specification').
The meiosis-specific SCP3 transcript was detected in treated cells after 14 days just like the germ cell specific transcript for DDX4 protein and Stella as a differentiation and maturation agent of PGCs, which is known to be expressed in post-migratory primordial germ cells until the postmeiotic stage of PGC like cells.
"For the first time in the world, Iranian and Canadian scientists succeeded in creating PGC from the skin of an infertile person," Karim Nayernia, an Iranian geneticist, told FNA.
When transplanted into mice that were unable to produce sperm normally, the stem cell derived PGCs produced normal-looking sperm, which were then used to successfully fertilize eggs.
Minitubules formed by the transplant of the PGCs have similar organization inside the seminiferous epithelium of recipient mice.
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