PGEMPlan de Gestion de l’Espace Maritime (French: Maritime Area Management Plan)
PGEMProstaglandin E2 Metabolite
PGEMProfessional Group on Engineering Management (IEEE)
PGEMPortland General Electric Merchant (Portland, OR)
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References in periodicals archive ?
The DNA pieces were then inserted into pGEM T-Easy vector that included an ampicillin resistant gene and a LacZ reporter gene.
(NYSE: PGEM) and US-based investment firm Clayton, Dubilier and Rice have inked a definitive agreement under which CD and R funds will acquire all of the outstanding shares of Ply Gem common stock in a go-private transaction valued at approximately USD 2.4bn, the companies said.
Reamplified PCR products from three individuals were then ligated into pGEM T-Easy (Promega) according to manufacturer instructions.
PCR products were subcloned into pGEM T-easy vector (Pro mega, Madison, WI) and sequenced on Li-Cor and ABI 3700 sequencing machines in the Josephine Bay Paul Center.
Several products were amplified by PCR, subcloned into pGEM T Easy vector (Promega), and clones were taken to prepare plasmids (minipreps).
The ISs, designed to be smaller than the corresponding target sequences, were homologous recombinant cDNAs obtained by oligonucleotide-mediated mutagenesis (13) from AHR, ARNT, CYP1B1, and [beta] -ACT sequences inserted in pGEM plasmid.
The PCR products were confirmed by electrophoresis on 0.8% agarose gel, after which they were cloned into the pGEM T-easy vector (Promega, USA).
CEP-coated fused-silica capillaries provided with the pGEM Double Stranded DNA Analysis Kit (Hewlett-Packard) with a 40-cm effective length and 48.5-cm total length were used.
Using the pGEM T-easy vector, DNase I (RNase-free) and M-MLV reverse transcriptase from TaKaRa Dalian (Dalian, China), primers were synthesized (Table 1) and PCR products were sequenced by AuGCT Biotechnology (Bejing, China).
We inserted a 608-bp amplification product of CYP1B1 cDNA in pGEM; we then deleted a 98-bp portion from the CYP1B1 sequence by oligonucleotide-mediated mutagenesis (16,17) as shown in Fig.
CEP-coated fused-silica capillaries provided with the pGEM Double Stranded DNA Analysis Kit (HewlettPackard) of 56 cm effective length and 64.5 cm total length were used.
Besides, pGEM T Easy vector (Promega, WI, USA) was used to transcript the probes.