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JUST and PGFP pointed out they were also aware that throughout the investigation, attempts to obtain independently verifiable data had not been successful and these include certified copies of communications between Ukrainian air traffic controllers and the flight crew on board the ill-fated airline and the Comma Separated Variable (CSV) file from the plane's flight data recorder.
For the reporter assay, plasmids expressing hRXR (10 ng), pGFP (10 ng), the relevant PPAR isoform (10 ng), and the corresponding reporter plasmid (30 ng) were cotransfected using FuGENE 6 transfection reagent (Roche) according to the manufacturer's instructions.
Control dsRNA was prepared from a 239-nucleotide fragment (394-620) of the green fluorescent protein gene (X83959) amplified from the pGFP cloning plasmid DNA vector.