PGK1Phosphoglycerate Kinase 1
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5]; HMBS, hydroxymethylbilane synthase [NT_033899 and NM_000190]; PGK1, phosphoglycerate kinase 1 [NM_000291]; PMM1, phosphomannomutase 1 [NM_002676]; PSMB6, proteasome (prosome, macropain) subunit, beta type, 6 [NM_002798.
To verify the presence of intact RNA, we amplified PGK1 and PAX8 (exons 5 and 6) in each sample under the conditions described above.
We also tested 5 housekeeping genes [LMNB1, lamin B1; EIF1AX, eukaryotic translation initiation factor 1A, X-linked; CASC3 (also known as MLN51), cancer susceptibility candidate 3; PPIA; and PGK1, phosphoglycerate kinase 1] as endogenous controls (data not shown).