The signet-ring cells were negative for synaptophysin, chromogranin A, and CD57 (PGP9
The reaction mixture for polymerase chain reaction (PCR) contained 2 x PCR reaction mixture buffer 10 [micro]L, cDNA 1 [micro]L and specific primers (20 [micro]m each primer), Oct4 and c-kit (annealing at 52[degrees]C, 35 cycles), THY-1 and PGP9
.5 (annealing at 56[degrees]C, 35 cycles).
Results: The highest PGP9
.5 (94.6[+ or -]0.4) and the lowest c-Kit positive (25.1[+ or -]0.7) in Percoll method was significantly (pL0.001) achieved in 32[degrees]0 percoll gradient.
There is variable expression of other neuroectodermal antigens including NSE (positive in this case), synaptophysin, neurofilament, GFAP and PGP9
This is the first demonstration of antibodies to pro-MMP-7 in cancer patients, although numerous circulating antitumor antibodies have been detected in cancer patient sera, with identified antigens such as Her2/neu (32), thymidine phosphorylase (16), and PGP9
Changes of protein gene product 9.5 (PGP9
.5) immunoreactive nerves in inflamed appendix.
CD 117, DOG 1, S100, CD34, chromogranin, synaptophysin, CD56, CD57, PGP9
.5, and desmin were all negative.
Primary rabbit polyclonal antisera against PGP9
.5 (ThermoFisher, Waltham, MA), a general cytoplasmic marker of neurons and certain neuroendocrine cells, and slides were incubated overnight at 4[degrees]C.
Endocrine cells in the GI and pancreatobiliary tracts and NETs are labeled by neuroendocrine markers, including synaptophysin, chromogranin A, CD56/NCAM1, Leu7/ B3GAT1, protein gene product 9.5 (PGP9
.5), and neuronspecific enolase.
Parafibromin, galectin-3, PGP9
.5, Ki67, and cyclin D1: using an immunohistochemical panel to aid in the diagnosis of parathyroid cancer.
Immunostains showed the tumour cells were negative for chromogranin, CK7, CK20, PGP9
.5 serotonin, glucagon, somatostatin, gastrin and insulin (Fig.