After placement of the test card that contains appropriate dry reagents and PIOP in the instrument, the card is warmed by a 37 [degrees]C heat source.
The ECT test cards were prepared from ecarin (Centerchem), calcium, PIOP, buffers, and stabilizers.
(5) LOT is the point at which the PIOP oscillation amplitude starts to increase again (Fig.
Dry chemistry plasminogen test cards were prepared by assembling the test card device and injecting 24 [micro]L of a solution [containing 1500 U/L thrombin, 500 000 U/L tissue plasminogen activator (t-PA), 500 000 U/L urokinase, PIOP, salts, buffers, and stabilizers] into the card chamber, freezing the card/reagent mixture, and lyophilizing the test card to yield the dry chemistry formulation.
Dry chemistry test cards suitable for screening patient and normal samples were prepared as follows: 24 [micro]L of a solution containing 1500 U/L thrombin, t-PA at a concentration of 200 000,400 000, 800 000, or 1000 000 U/L, PIOP, salts, buffers, and stabilizers were injected into an assembled test card device, frozen, and lyophilized to yield the dry chemistry formulation.