PIROCPersistence Impact Ratio on Classes
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Outer reactions were performed with BS1 (forward, 5'-GACGGTAGGGTATTGGCCT-3') and PiroC (reverse, 5'-CCAACAAAATAGAACCAAAGTCCTAC-3") primers (36 cycles of 1 min at 94[degrees]C, 1 min at 59[degrees]C, and 1 min at 72[degrees]C) and inner reactions with PiroA (forward, 5"-ATTACCCAATCCTGACACAGGG-3') according to Armstrong et al.