PIV2

AcronymDefinition
PIV2ParaInfluenza Virus Type 2
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Virus Number of specimens (+: positive; -: negative) PCR-/FA- PCR+/FA+ PCR+/FA- PCR-/FA+ ADV 71 0 0 3 229E 74 0 0 0 OC43 67 7 0 0 HKU1 73 1 0 0 NL63 73 1 0 0 MPV 72 2 0 0 HRV 65 3 0 6 FluA 66 8 0 0 FluB 72 2 0 0 PIV1 74 0 0 0 PIV2 74 0 0 0 PIV3 69 4 1 0 PIV4 74 0 0 0 RSV 59 10 2 3 Virus Sensitivity Specificity PPV NPV Kappa (%) (%) (%) (%) ADV 0 95.9 0 100 0 229E 0 100 0 100 1 OC43 100 100 100 100 1 HKU1 100 100 100 100 1 NL63 100 100 100 100 1 MPV 100 100 100 100 1 HRV 100 91.5 33.3 100 0.468 FluA 100 100 100 100 1 FluB 100 100 100 100 1 PIV1 0 100 0 100 1 PIV2 0 100 0 100 1 PIV3 80 100 100 98.6 0.882 PIV4 0 100 0 100 1 RSV 83.3 95.2 76.9 96.7 0.752 FA, FilmArray RP; PPV, positive predictive value; NPV, negative predictive value.
The uidA gene used in these experiments contained the PIV2 intron of the ST-L1 gene from Solanum tuberosum within its coding sequence (uidAint), thereby preventing its expression in Agrobacterium [35].
Transgenic cultures proliferated in selective medium and expressed the uidA gene carrying the PIV2 intron, thereby demonstrating its functionality.
The following year the second generation PIV2 (named City Bee) was designed and developed.
There were 309 children infected with PIVs, including PIV1 (0.9%, 69/8031), PIV2 (0.2%, 14/8031) and PIV3 (2.8%, 226/8031), which were mostly found in children aged 7-12 months (Table 1).
Virus Total Single Multiple N (%) N (%) N (%) Influenza Influenza A 63 18.75 27 42.86 36 57.14 Influenza B 58 17.26 25 43.10 33 56.90 Human rhinovirus 76 22.62 29 38.16 47 61.84 Human adenovirus 67 19.94 12 17.91 55 82.09 Respiratory syncytial virus RSVA 53 15.77 19 35.85 34 64.15 RSVB 4 1.19 1 25 3 75 Human enterovirus 36 10.71 13 36.11 23 63.89 Parainfluenza virus PIV1 12 3.57 2 16.67 10 83.33 PIV2 2 0.60 0 0 2 100 PIV3 5 1.49 4 80 1 20 PIV4 1 0.30 0 0 1 100 Human bocavirus 15 4.46 2 13.33 13 86.67 Human coronavirus Type NL63 6 1.79 2 33.33 4 66.67 Type OC43 4 1.19 1 25 3 75 Type 229 E 3 0.89 1 33.33 2 66.67 Table 2: Contribution of respiratory viruses as single or coinfections.
One case was found to have a triple infection of PIV2, IFVB, and HBoV.