Caption: Figure 4: Duodenal mass with metastatic seminoma (PLAP
IHC stain, magnification x200).
Previous research carried out on PLAP
has mainly focused on its effectiveness as a learning tool (Nkoma, 2012 & 2013), how children and parents have received PLAP
, (UNICEF, 2012) carried out in the secondary school sector, (MoESAC, 2012).
La elevada disminucion del peso molecular viscosimetrico de la fase PLA en el nanocompuesto (PLAm) con respecto al PLA procesado (PLAp
) se puede atribuir a la presencia de la nanocarga en el sistema (79% de reduccion del Mv con respecto al PLA sin procesar).
* Administer PLAP
on a sample, not a census, basis;
Leydig cell tumors are also positive for calretinin, Melan A, and vimentin and are negative for germ cell markers such as CD117, OCT3/4, PLAP
, AFP, and [beta]-HCG.
Dysgerminoma is said to show a 100% positivity for PLAP
, but recent studies counter this.
Immunohistochemical staining results showed inhibin (+), CD99 (focal+), CD117 (focal+), Ki-67 (20%+), PLAP
(focal+), OCT-4 (focal+), D2-40 (focal+), GPC3 (−), AFP (−), CD30 (−), and CK (focal+).
Although placental alkaline phosphatase (PLAP
) CD-117 and AFP immunohistochemical stains were negative the diagnosis of Primary Mediastinal Germ Cell Tumour (PMGCT) was made as this was the highest possibility at this young age with raised AFP.
Les marqueurs immunohistochimiques classiques des tumeurs du sac vitellin comprennent notamment l'[alpha]FP, la phosphatase alcaline placentaire (PLAP
) et le glypican-3.
The tumor was negative for CKAE1/AE3, NSE, CD45, PLAP
, CD117, S-100, Chromogranin, Calretinin, TTF-1, CD34, and EMA.
Final histopathological analysis revealed a classic seminoma, which stained positive for placental alkaline phosphatase (PLAP
), but negative for Ber-H21, inhibin and Alphafeto protein (Fig.
In tumour tissue, immunoreaction was observed only by vimentin, but no expressions were observed with pankeratin, epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA), cytokeratin-7, cytokeratin-20, leukocyte common antigen (LCA), smooth muscle actin (SMA), desmin, Myo-D1, chromogranin-A, synaptophysin, S100 protein, HMB45, CD68, CD30, CD34, CD31, factor VIII, alpha feto protein (AFP), beta human chorionic gonadotropin ((beta) hCG), placental alkaline phosphatase (PLAP
), and Neu-N.