analysis showed an increased fungal; bacterial ratio with rehabilitation time, which is indicative of improved nutrient cycling ability within the soil system (Courtney et al.
Another set was divided into two parts: for PLFA
analysis, kept at -20[degrees]C; for parameter analyses, dried in shade, homogenized, and sieved before preserved.
Three frequently used fingerprinting techniques that generate these multivariate datasets are phospholipid fatty acid analysis (PLFA
), denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism analysis (TRFLP).
Assessing shifts in microbial community structure across a range of grasslands of differing management intensity using CLPP, PLFA
and community DNA techniques.
must now find pounds 7,000 in the next month to secure the review.
For each sample collected on the final sampling date (5 March 1997), the microbial community structure was characterized by determining the phospholipid fatty acid (PLFA
Microbial community structure, as revealed by the PLFA
pattern, changed linearly along the soil gradient and was modified by earthworm presence and by time [ILLUSTRATION FOR FIGURE 6A, B OMITTED].
Several approaches used to characterize soil microorganisms, such as soil microbial biomass, Biolog carbon source substrate utilization, soil phospholipid fatty acid (PLFA
) and PCR-denaturing gradient gel electrophoresis (PCR-DGGE) have been developed to evaluate the soil quality.
Soil microorganisms were monitored by determination of dehydrogenase activity and respiration, known to be indicative of proceeding biodegradation , and phospholipid fatty acid (PLFA
) content, common method for estimation of living microbial biomass in soils [18,19], sediments , and other matrices .
The biomass of bacteria and fungi was estimated by measuring phospholipid fatty acid (PLFA
) concentration in soil (method presented by Frostegard et al.
Our results are also in agreement, while using phospholipid fatty acid (PLFA
) analysis (Fig.
We used phospholipid fatty acid (PLFA
) analysis to examine the relation of microbial biomass and community composition to vegetation zonation on a coastal sand dune.