The PLHC-1 bioassay procedures were a slight modification of the H4IIE bioassay methods described in Tillitt et al.
All SPMD extracts induced ethoxyresorufin-O-deethylase (EROD) activity in the PLHC-1 cells.
Retene induced EROD in PLHC-1 at concentrations of about 30-600 ng/well.
It is unknown how potent these compounds are as MFO inducers in PLHC-1.
The abundance of C1- to C3-substituted PAHs in the Norman Wells SPMDs indicates that substituted PAHs may have been present in the stormwater-exposed SPMDs, and these may have accounted for some of the observed induction in PLHC-1 in the Villeneuve et al.