Key words: semipermeable membrane devices (SPMDs), mixed function oxygenase (MFO), Ethoxyresorufin-O-deethylase (EROD), oil refinery effluent, Mackenzie River, Norman Wells, cell line, PLHC-1, polyaromatic hydrocarbons (PAHs)
Extracts of SPMDs were tested for potency of MFO induction in a fish (Poeciliopsis lucida, a top minnow) hepatoma cell line, PLHC-1. Increased EROD activity in PLHC-1 indicated that MFO inducers were present in the SPMD extracts; therefore, they were present in the effluent or river water and were potentially bioavailable to fish.
The extracts were dosed to fish liver cells (PLHC-1, Poeciliopsis lucida hepatoma cells, a liver tumour cell line from a top minnow) for 72 h.
SPMD extracts were tested for EROD induction potency in Poeciliopsis lucida hepatoma cells (PLHC-1).
All SPMD extracts induced ethoxyresorufin-O-deethylase (EROD) activity in the PLHC-1 cells.
Retene induced EROD in PLHC-1 at concentrations of about 30-600 ng/well.
It is unknown how potent these compounds are as MFO inducers in PLHC-1. Chrysene (30 mg/kg, i.p.
The abundance of C1- to C3-substituted PAHs in the Norman Wells SPMDs indicates that substituted PAHs may have been present in the stormwater-exposed SPMDs, and these may have accounted for some of the observed induction in PLHC-1 in the Villeneuve et al.