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Electrolyte leakage in samples in packages which had 21.4 and 29.5 pmol [s.sup.-1] [m.sup.-2] [Pa.sup.-1] OTR did not increase significantly by the end of the 25-day storage period (P < 0.01) compared to the starting values.
In order to optimize transfection of freshly isolated [CD105.sup.+] cells, polyplexes with different N/P ratios (N/P 2.5, N/P 10, and N/P 33) and miR amounts (5 pmol and 10 pmol) were tested using flow cytometry.
Magnetic polyplexes consisting of an N/P ratio of 10 combined with 5 pmol miR had similar uptake rates (up to 76%) compared to the corresponding miR/PEI complexes (81%) without the application of a magnetic field (Figure 3(a)).
miR/PEI/MNP complexes with an N/P ratio of 10 combined with 5 pmol miR bound to 1 [micro]g/mL MNPs showed high uptake rates (68%, Figure 4(a)).
brasiliana is attracted to a non-laying conspecific and displays behaviors suggestive of mating when 10 pmol of attractin is placed in the adjacent artificial seawater, even though attractin is a product of the A.
brasiliana with 1 pmol of either native or recombinant attractin in the adjacent ASW to a non-laying conspecific with nothing added.
The response pattern changed when 1 pmol of either native or recombinant attractin was placed in the seawater adjacent to the stimulus animal (Fig.
The rates measured ranged from 173 pmol oxygen [larva.sup.-1] [h.sup.-1] to 261 pmol oxygen [larva.sup.-1] [h.sup.-1], with a mean rate of 213 ([+ or -]35, 95% CI) pmol [larva.sup.-1] [h.sup.-1] for the six independent measurements.
Plantigrades (at 20 [degrees] C, Culture D) 3 days after settlement (stage iv) had a 3-fold higher maximal transport capacity for alanine ([J.sub.max]) = 182.0 [+ or -] 49.2 [SE] pmol [individual.sup.-1] [h.sup.-1], [K.sub.t] = 96 [[micro]molar]) than that of the stage ii veligers ([J.sub.max] = 61.2 [+ or -] 1.9 pmol [individual.sup.-1] [h.sup.-1], [K.sub.t] = 29 [[micro]molar]) [ILLUSTRATION FOR FIGURE 3A, B OMITTED].
For the nonspecific competitor DNA assays, everything was identical except labeled F-DNA and Ape1 were present at 0.1 pmol and 50 pg, respectively.
DNA substrate (1 pmol) was added after a 10-min incubation on ice, and the incision efficiency was determined as for Ape1 (see above).
PRL concentrations in untreated and treated samples were 215 [micro]g/L (9350 pmol/L) and 200 [micro]g/L (8696 pmol L), respectively; therefore, no notable amount of macro-PRL was detected.
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