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5, 150 mM NaCl, 1% v/v Igepal CA-630, 1 mM PMSF, 1 mM sodium fluoride, and 10 [micro]g/mL aprotinin and leupeptin).
66 TABLE II EFFECT OF PROTEASE INHIBITORS AND METAL IONS ON THE ACTIVITY OF TRYPSIN FROM Dosidicus gigas Inhibitor/Ion Concentration Residual activity (%) Control (No inhibitor/Ion) 100 PMSF 100 mM 2 [+ or -] 0.
Both proteinases were inhibited by PMSF and identified as serine proteinases (Fig.
1 Inhibition (a) (%) Extracts Lean Liver Fatty liver Inhibitors PMSF EDTA Plain homogenate 17.
500 ng of the membrane protein resuspended in PBS containing 1 mM PMSF and 1 mM aprotinin.
SBTI (soybean trypsin inhibitor), TPCK (N-tosyl-L-phenyl -chloromethyl ketone), PMSF (phenylmethylsulfonyl fluoride), TLCK (N[alpha]-tosyl-L-lysine chloromethyl ketone hydrochloride), Phen (1.
La suspension de CM-S, con histonas unidas, se lavo cuatro veces mas con NaCl 0,35 M, Tris 10 mM, PMSF 0,1 mM a pH 8,8 para retirar todos los restos.
Presence of serine proteases; CTRL indicates the proteolytic activity at pH 8 in a normal reaction, whereas the PMSF lane indicates the activity after incubating the sample with this specific inhibitor for serine proteases.
05% Triton X-100] containing inhibitor cocktail and PMSF in cells.
25% Na-deoxycholate 150 mM NaCl 1 mM EDTA 1 mM PMSF 1 mM Na3VO4 1 mM NaF and protease inhibitors (Complete Mini; Roche Switzerland)].
As the protease activity was inhibited by phenylmethylsulfonylfluoride (PMSF), the mycelia cultured at 25 [degrees]C for 70 hr were homogenized at 4 [degrees]C in the presence of PMSF and purified.
The supernatant was collected from the culture broth by centrifugation at 10,000 g for 20 min and after that the pellet was resuspended in 1 mM EDTA, 1 mM PMSF, I mM DTT, and 5% glycerol in 50 mM potassium phosphate buffer (pH=7.
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