As shown in Figure 7, the expression of 7 genes of PP2C and SAPK3 were upregulated, and a PYL4 gene was suppressed in ABA-treated hemp plants (Figure 7), which was in accordance with analysis obtained from drought-stressed hemp.
In our study, eight genes in ABA biosynthesis were noticeably influenced by drought stress, including PYL4, PP2C, and SAPK3 (Figure 6).
[Mg.sup.2+] is an activator of PP2C, and SC has recently been shown to potently and specifically inhibit PP2C (15).
The combined activity of CN and PP2C (inhibition by O A only) was 55.1 (8.8), with PP2C activity alone (OA and EGTA inhibition) being 8.7 (0.9).
Serine/threonine-specific phosphatases can be divided into two major families: phospho protein phosphatase (PPP) members, which include type 1 (PP1), 2A (PP2A) and 2B (PP2B/calcineurin); and protein phosphatase magnesium-dependent (PPM) members, which include protein phosphatase 2C (PP2C) and require [Mg.sup.2+] or [Mn.sup.2+] for activity (Cohen 1989; Klumpp et al.
On the other hand, it is known that oleic acid and ginkgolic acid activate PP2C, although they also inhibit PP1, PP2A and PP2B (Klumpp et al.
Under normal conditions, proteins called PP2Cs inhibit the ABA pathway, but when a plant is subjected to drought, the concentration of ABA in its cells increases.
But, ABA does not interact directly with PP2Cs, so how does it cause them to be inhibited?
domestica) 2 AJ277744 807 Proteina 3 x [10.sup.-25] fosfatase 2C (PP2C
) (Fagus sylvatica) 2 e 5 AJ507459 355 Profilina 5 x [10.sup.-43] alergenica (M.
From the test samples, the background phosphatase activity (0 U from the calibration curve) was subtracted, leading to the activity attributable to CaN and other okadaic acid-resistant phosphatases (PP2C
"Binding" (14,364, 21.11%) was the second most prevalent category and included ARF and BRI1, followed by "metabolic processes" (14,261, 20.96%), including ARF, pyrabactin resistance/ pyrabactin resistance-like (PYR/PYL), protein phosphatase 2C (PP2C
), ethylene-insensitive protein 2 (EIN2), ABF, and TGA.
The remaining phosphatase activity can then be attributed to CN and PP2C
, the latter being CsA-resistant.