Out of total 900 cultured samples 176 (19.55%) showed mass turbidity and whirling movement in PPLO
broth, which indicated positive growth of Mycoplasma.
Samples inoculated in mycoplasma growth medium (PPLO
broth) showed whorl formation with turbidity which was detected in the presence of light.
With culture method, mycoplasma isolation was not achieved from any of these samples in a tube with PPLO
liquid culture medium and/or PPLO
solid culture media.
A total of 300 samples (the nasal discharge, pleural fluid and lung tissues) were collected at farms, herds and abattoirs from those suspected of CCPP, and were transferred to DifcoTM PPLO
broth (Becton Dickinson, Sparks, MD, USA), and transported in ice box to our Mycoplasma reference laboratory at the Faculty of Animal and Veterinary Science, University of Agriculture, Peshawar.
The specimens were incubated in PPLO
broth, enriched with 5% C[O.sub.2] in humid air at 37[degrees]C for 8-16 hours.
Policarpio said the PPLO
should have informed the President that there was an intense Alvarez-Arroyo power but it decided to sit beside Alvarez at the Speaker's office.
Lactose, raffinose, and trehalose fermentation was assessed, using [S4074.sup.T] and [NM305.sup.T] as control strains, in PPLO
broth (Difco) supplemented with 40 [micro]g/ml NAD, as described previously [1, 8].