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The sera samples collected from clinically recovered animals were analyzed for the presence of PPRV specific antibodies.
There has been a substantial improvement in the techniques to detect the nucleic acids of PPRV. PCR assays are now considered as powerful as well as novel means of detection and quantification of the nucleic acids of PPR virus in various types of clinical samples.
The PPRV circulation is proven towards this survey but none vaccination program was applied.
No serologic and clinical reports of PPRV infection in wildlife occurred in sub-Saharan Africa during 2005-2013, although seropositivity was recorded in Uganda, Ethiopia, and other countries in West and Central Africa before this period (4).
Thus, it appears that goats were more susceptible to PPRV infection than sheep.
The new PPRV-LAMP setup excludes the need for pre-requisite steps of total RNA extraction and reverse transcription prior to the amplification of target viral genome and post amplification lengthy analysis by gel electrophoresis, thereby, providing with the option of field based sensitive, reliable and efficient molecular diagnosis of PPRV.Thesensitivity and reliability of the LAMP was compared with conventional RT-PCR.
PPRV has caused numerous serious epidemics in small ruminant populations across sub-Saharan Africa, the Middle East, and major parts of the Indian subcontinent where PPRV is considered endemic (1).
The serum samples showing PI value of 50 or above were taken as positive for PPRV antibodies, while those below value of 50 were considered negative.
Historically, PPRV has been identified across much of the developing world; genetic analyses has grouped viruses into 4 lineages that were originally thought to be phylogeographically restricted (6).
A unique serotype of PPRV circulates and is classified into 4 genetically distinct lineages (3).
At multiplicity of infection (MOI) of 0.1 83.6 percent of PBMCs can be infected with PPRV. PPRV could completely inhibit lymphoproliferation at MOI of 0.75 while NPPRV and 420-525 NPPRV could suppress lymphoproliferation by 7.4 % and 17.6 % respectively.
PPRV was the single viral antigen (Figure 2a) in 15 (10%) animals.
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